Fluorescent protein-based Zn²⁺ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn².

ABSTRACT: Zinc ions are required by all known organisms. Maintaining zinc homeostasis by preventing toxic overload while ensuring sufficient acquisition for cellular functions is crucial for survival and growth of bacteria. Bacteria, however, frequently encounter and must survive in various environments. During infection in host animals, for example, bacteria are exposed to acidic conditions in the stomach and anaerobic conditions in the intestines, but the effects of oxygen on zinc homeostasis in Escherichia coli have not been well-studied. Previously, we reported a flavin-binding fluorescent protein-based zinc sensor, CreiLOV_N41C, which can respond to changes in labile Zn²⁺ levels in bacteria under both aerobic and anaerobic conditions. Here, we combined the use of CreiLOV

SUBMITTER: Nguyen HN 

PROVIDER: S-EPMC11550654 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature