Therapeutic efficacy of SipD/LptD-specific IgY entrapped in alginate nanoparticles against Salmonella Typhimurium infection.

ABSTRACT:

Introduction

Salmonella, a zoonotic pathogen causing gastroenteritis, lacks a preventive vaccine. Passive immunization with IgY antibodies derived from immunized chickens has shown potential for treating bacterial infections. This study investigated the therapeutic efficacy of entrapped IgY targeting recombinant SipD and LptD proteins from Salmonella Typhimurium.

Methods

The recombinant protein was expressed in E. coli BL21 (DE3) and purified using Ni-NTA affinity chromatography. Hens were immunized with the purified protein, and the resulting IgY was entrapped into alginate nanoparticles. The shape of spherical nanoparticles and their size in the nanometer range were determined by SEM and DLS analysis. The therapeutic efficacy of free and alginate-entra

SUBMITTER: Zafarmand-Samarin M 

PROVIDER: S-EPMC11550739 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature