Bessel beam optical coherence microscopy enables multiscale assessment of cerebrovascular network morphology and function.

ABSTRACT: Understanding the morphology and function of large-scale cerebrovascular networks is crucial for studying brain health and disease. However, reconciling the demands for imaging on a broad scale with the precision of high-resolution volumetric microscopy has been a persistent challenge. In this study, we introduce Bessel beam optical coherence microscopy with an extended focus to capture the full cortical vascular hierarchy in mice over 1000 × 1000 × 360 μm³ field-of-view at capillary level resolution. The post-processing pipeline leverages a supervised deep learning approach for precise 3D segmentation of high-resolution angiograms, hence permitting reliable examination of microvascular structures at multiple spatial scales. Coupled with high-sensitivity Doppler optical coherenc

SUBMITTER: Glandorf L 

PROVIDER: S-EPMC11551179 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature