Perturbation-specific transcriptional mapping for unbiased target elucidation of antibiotics.

ABSTRACT: The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for antibiotic discovery are being developed, target elucidation of new chemical entities remains challenging. In the postgenomic era, expression profiling can play an important role in mechanism-of-action (MOA) prediction by reporting on the cellular response to perturbation. However, the broad application of transcriptomics has yet to fulfill its promise of transforming target elucidation due to challenges in identifying the most relevant, direct responses to target inhibition. We developed an unbiased strategy for MOA prediction, called perturbation-specific transcriptional mapping (PerSpecTM), in which large-throughput expression profiling of wild-type or hypomorphic mutants, depl

SUBMITTER: Romano KP 

PROVIDER: S-EPMC11551328 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature