SUPREM: an engineered non-site-specific m6A RNA methyltransferase with highly improved efficiency.

ABSTRACT: N 6-Methyladenine (m6A) RNA methylation plays a key role in RNA processing and translational regulation, influencing both normal physiological and pathological processes. Yet, current techniques for studying RNA methylation struggle to isolate the effects of individual m6A modifications. Engineering of RNA methyltransferases (RNA MTases) could enable development of improved synthetic biology tools to manipulate RNA methylation, but it is challenging due to limited understanding of structure-function relationships in RNA MTases. Herein, using ancestral sequence reconstruction, we explore the sequence space of the bacterial DNA methyltransferase EcoGII (M.EcoGII), a promising target for protein engineering due to its lack of sequence specificity and its residual activity on RNA. We thereby c

SUBMITTER: Ochiai Y 

PROVIDER: S-EPMC11551740 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature