Establishing a dominant early larval sex-selection strain in the Asian malaria vector Anopheles stephensi.

ABSTRACT:

Background

Genetic biocontrol interventions targeting mosquito-borne diseases require the release of male mosquitoes exclusively, as only females consume blood and transmit pathogens. Releasing only males eliminates the risk of increasing mosquito bites and spreading pathogens while enabling effective population control. The aim of this study is to develop robust sex-sorting methods for early larval stages in mosquitoes, enabling scalable male-only releases for genetic biocontrol interventions.

Methods

To address the challenge of sex-sorting in the Asian malaria vector Anopheles stephensi, we engineer Sexing Element Produced by Alternative RNA-splicing of a Transgenic Observable Reporter (SEPARATOR). This dominant fluorescent-based method, previously proven effective in Aede

SUBMITTER: Weng SC 

PROVIDER: S-EPMC11552218 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature