Dissecting thrombus-directed chemotaxis and random movement in neutrophil near-thrombus motion in flow chambers.

ABSTRACT:

Background

Thromboinflammation is caused by mutual activation of platelets and neutrophils. The site of thromboinflammation is determined by chemoattracting agents release by endothelium, immune cells, and platelets. Impaired neutrophil chemotaxis contributes to the pathogenesis of Shwachman-Diamond syndrome (SDS). In this hereditary disorder, neutrophils are known to have aberrant chemoattractant-induced F-actin properties. Here, we aim to determine whether neutrophil chemotaxis could be analyzed using our previously developed ex vivo assay of the neutrophils crawling among the growing thrombi.

Methods

Adult and pediatric healthy donors, alongside with pediatric patients with SDS, were recruited for the study. Thrombus formation and granulocyte movement in hirudinated whole

SUBMITTER: Korobkin JD 

PROVIDER: S-EPMC11552338 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature