Comparative transcriptomic analyses of thymocytes using 10x Genomics and Parse scRNA-seq technologies.

ABSTRACT:

Background

Single-cell RNA sequencing experiments commonly use 10x Genomics (10x) kits due to their high-throughput capacity and standardized protocols. Recently, Parse Biosciences (Parse) introduced an alternative technology that uses multiple in-situ barcoding rounds within standard 96-well plates. Parse enables the analysis of more cells from multiple samples in a single run without the need for additional reagents or specialized microfluidics equipment. To evaluate the performance of both platforms, we conducted a benchmark study using biological and technical replicates of mouse thymus as a complex immune tissue.

Results

We found that Parse detected nearly twice the number of genes compared to 10x, with each platform detecting a distinct set of genes. The comparison of

SUBMITTER: Filippov I 

PROVIDER: S-EPMC11552371 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature