Real-Time Spectral Library Matching for Sample Multiplexed Quantitative Proteomics.

ABSTRACT: Sample multiplexed quantitative proteomics assays have proved to be a highly versatile means to assay molecular phenotypes. Yet, stochastic precursor selection and precursor coisolation can dramatically reduce the efficiency of data acquisition and quantitative accuracy. To address this, intelligent data acquisition (IDA) strategies have recently been developed to improve instrument efficiency and quantitative accuracy for both discovery and targeted methods. Toward this end, we sought to develop and implement a new real-time spectral library searching (RTLS) workflow that could enable intelligent scan triggering and peak selection within milliseconds of scan acquisition. To ensure ease of use and general applicability, we built an application to read in diverse spectral libraries and file

SUBMITTER: McGann CD 

PROVIDER: S-EPMC11554524 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature