Jag1 insufficiency alters liver fibrosis via T cell and hepatocyte differentiation defects.

ABSTRACT: Fibrosis contributes to tissue repair, but excessive fibrosis disrupts organ function. Alagille syndrome (ALGS, caused by mutations in JAGGED1) results in liver disease and characteristic fibrosis. Here, we show that Jag1^Ndr/Ndr mice, a model for ALGS, recapitulate ALGS-like fibrosis. Single-cell RNA-seq and multi-color flow cytometry of the liver revealed immature hepatocytes and paradoxically low intrahepatic T cell infiltration despite cholestasis in Jag1^Ndr/Ndr mice. Thymic and splenic regulatory T cells (Tregs) were enriched and Jag1^Ndr/Ndr lymphocyte immune and fibrotic capacity was tested with adoptive transfer into Rag1^-/- mice, challenged with dextran sulfate sodium (DSS) or bile duct ligation (BDL). Transplanted Jag1^Ndr/Ndr l

SUBMITTER: Masek J 

PROVIDER: S-EPMC11554675 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature