A novel approach to double-strand DNA break analysis through γ-H2AX confocal image quantification and bio-dosimetry.

ABSTRACT: DNA damage occurs in all living cells. γ-H2AX imaging by fluorescent microscopy is widely used across disciplines in the analysis of double-strand break (DSB) DNA damage. Here we demonstrate a method for the quantitative analysis of such DBSs. Ionising radiation, well known to induce DSBs, is used in this demonstration, and additional DBSs are induced if high-Z nanoparticles are present during irradiation. As a deliberate test of the methodology, cells are exposed to a spatially fractionated ionising radiation field, characterised by regions of high and low absorbed radiation dose that are only ever qualitatively verified biologically via γ-H2AX imaging. Here we validate our bio-dosimetric quantification method using γ-H2AX assays in the assessment of DSB enhancement. Our method reliably q

SUBMITTER: Valceski M 

PROVIDER: S-EPMC11554680 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature