Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential.

ABSTRACT:

Background

As fat grafting is commonly used as a filler, Adipose-derived stem/stromal cells (ASC) have been reported to be key player in retention rate. Paracrine and differentiation potential of those cells confer them strong pro-angiogenic capacities. However, a full characterization of the influence of aging on ASC has not been reported yet. Here we've investigated the effect of age on paracrine function, stemness and angiogenic potential of ASC.

Methods

ASC were extracted from young and old adult donors. We assessed stromal vascular fraction cell populations repartition, ASC stemness potential, capability to differentiate into mesenchymal lineages as well as their secretome. Angiogenic potential was assessed using a sprouting assay, an indirect co-culture of ASC and derm

SUBMITTER: Trotzier C 

PROVIDER: S-EPMC11555058 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature