Revealing single-neuron and network-activity interaction by combining high-density microelectrode array and optogenetics.

ABSTRACT: The synchronous activity of neuronal networks is considered crucial for brain function. However, the interaction between single-neuron activity and network-wide activity remains poorly understood. This study explored this interaction within cultured networks of rat cortical neurons. Employing a combination of high-density microelectrode array recording and optogenetic stimulation, we established an experimental setup enabling simultaneous recording and stimulation at a precise single-neuron level that can be scaled to the level of the whole network. Leveraging our system, we identified a network burst-dependent response change in single neurons, providing a possible mechanism for the network-burst-dependent loss of information within the network and consequent cognitive impairment during e

SUBMITTER: Kobayashi T 

PROVIDER: S-EPMC11555060 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature