Zinc finger protein 296 promotes hepatocellular carcinoma progression via intervening interaction between macrophages and B cells.

ABSTRACT:

Objective

Hepatocellular carcinoma (HCC) is a prevalent malignancy with poor survival. Different cell types in the tumor microenvironment participate in the tumorigenesis and progression of HCC. This study aimed to analyze the immune microenvironment of HCC and its relationship with clinical outcomes.

Methods

We analyzed HCC RNA-seq for cell type identification and prognosis by estimating relative subsets of RNA transcripts using CIBERSORTx. The interaction between B cells and macrophages in HCC was analyzed using a Hepa1-6 orthotopic transplantation mouse model and flow cytometry. The effect of Zinc finger protein 296 (ZNF296) on the interaction of B cells and macrophages was verified using human HCC tissues analyzed through western blot, quantitative real-time polymerase c

SUBMITTER: Xu N 

PROVIDER: S-EPMC11555204 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature