Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models.

ABSTRACT: 3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and d

SUBMITTER: Avelino TM 

PROVIDER: S-EPMC11555214 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature