Unraveling devitalization: its impact on immune response and ectopic bone remodeling from autologous and allogeneic callus mimics.

ABSTRACT: Endochondral bone regeneration is a promising approach in regenerative medicine. Callus mimics (CMs) are engineered and remodeled into bone tissue upon implantation. The long-term objective is to fabricate a sustainable off-the-shelf treatment option for patients. Devitalization was introduced to facilitate storage and using allogeneic (donor) cells would further propel the off-the-shelf approach. However, allogeneic CMs for bone regeneration pose a potential antigenicity concern. Here, we explored the impact of devitalization on antigenicity and osteoinductive bone formation when implanting syngeneic or allogeneic CM in a vital or devitalized state. For this, we implanted chondrogenically differentiated rat-derived mesenchymal stromal cells using an allogeneic immunocompetent ectopic rat

SUBMITTER: de Silva L 

PROVIDER: S-EPMC11555479 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature