Comparison of Protocols to Test Peptide Stability in Blood Plasma and Cell Culture Supernatants.

ABSTRACT: Due to their high specificity, peptides are promising candidates in drug development, but fast degradation often limits their biological activity. Thus, a short half-life is one of the major challenges in the development of new peptide therapeutics. Moreover, the enzymatic cleavage of peptides can be a reason for misleading results in biological assays. Peptide stability assays typically consist of incubation, precipitation, and detection steps. However, the current methods differ greatly regarding these three steps, thus limiting the compatibility. Here, we systematically evaluate different parameters of peptide stability assays. First, we quantified and compared the analyte loss during the precipitation of plasma proteins. Especially, broadly used precipitation by strong acids was found

SUBMITTER: Kohler A 

PROVIDER: S-EPMC11555501 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature