Cordycepin improves hyperactivation and acrosome reaction through adenosine receptors during human sperm capacitation in vitro.

ABSTRACT:

Background

Sperm capacitation is a prerequisite for natural or in vitro fertilization. After capacitation, sperm become hyperactivated and undergo an acrosome reaction, which helps them penetrate the oocyte. Cordycepin, a bioactive compound first isolated from Cordyceps militaris, is an adenosine analog with numerous physiological activities. However, its effects on sperm capacitation remain unclear. This study aims to elucidate the effects and mechanisms of cordycepin on human sperm capacitation.

Methods

During in vitro capacitation culture, healthy human sperm were treated with cordycepin (20, 100, 500 µM). Sperm motility and hyperactivation were detected using a computer-assisted sperm analyzer. Sperm acrosome reaction was measured using fluorescein isothiocyanate-conjuga

SUBMITTER: Shan L 

PROVIDER: S-EPMC11555834 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature