Efficient DNA-free co-targeting of nuclear genes in Chlamydomonas reinhardtii.

ABSTRACT: Chlamydomonas reinhardtii, a model organism for unicellular green microalgae, is widely used in basic and applied research. Nonetheless, proceeding towards synthetic biology requires a full set of manipulation techniques for inserting, removing, or editing genes. Despite recent advancements in CRISPR/Cas9, still significant limitations in producing gene knock-outs are standing, including (i) unsatisfactory genome editing (GE) efficiency and (ii) uncontrolled DNA random insertion of antibiotic resistance markers. Thus, obtaining efficient gene targeting without using marker genes is instrumental in developing a pipeline for efficient engineering of strains for biotechnological applications. We developed an efficient DNA-free gene disruption strategy, relying on phenotypical identification o

SUBMITTER: Battarra C 

PROVIDER: S-EPMC11556018 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature