Dissection of signaling pathways regulating TrkB-dependent gephyrin clustering.

ABSTRACT:

Introduction

The TrkB receptor is known for its role in regulating excitatory neuronal plasticity. However, accumulating evidence over the past decade has highlighted the involvement of TrkB in regulating inhibitory synapse stability and plasticity, particularly through regulation of the inhibitory scaffold protein gephyrin, although with contradicting results.

Methods

In this study, we extended on these findings by overexpressing rat TrkB mutants deficient in either Shc-or PLCγ-dependent signaling, as well as a kinase-dead mutant, to dissect the contributions of specific TrkB-dependent signaling pathways to gephyrin clustering.

Results

Our results demonstrate that TrkB signaling is required for gephyrin clustering on the perisomatic area of granule cells in the denta

SUBMITTER: Wustner LS 

PROVIDER: S-EPMC11556255 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature