Comparison of the protein composition of isolated extracellular vesicles from mouse brain and dissociated brain cell culture medium.

ABSTRACT: Extracellular vesicles (EVs) play a crucial role in intercellular communication. Characterizing EV protein composition is essential to understand EV function(s). Isolating EVs from cell culture medium is a common approach to study EVs, but it remains unclear whether EVs isolated from in vitro conditions accurately reflect physiological conditions of the same source in vivo tissues. Here, we analyzed the protein composition of EVs isolated from freshly dissected mouse forebrain and primary dissociated mouse forebrain culture medium. In total, 3,204 and 3,583 proteins were identified in EVs isolated in vivo and in vitro, respectively. Among the proteins identified from both EV sources, there was substantial overlap (~86%). While the overall proteome compositions were very similar, in vitro E

SUBMITTER: Xu Z 

PROVIDER: S-EPMC11556680 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature