An automated high-content screening and assay platform for the analysis of spheroids at subcellular resolution.

ABSTRACT: The endomembrane system is essential for healthy cell function, with the various compartments carrying out a large number of specific biochemical reactions. To date, almost all of our understanding of the endomembrane system has come from the study of cultured cells growing as monolayers. However, monolayer-grown cells only poorly represent the environment encountered by cells in the human body. As a first step to address this disparity, we have developed a platform that allows us to investigate and quantify changes to the endomembrane system in three-dimensional (3D) cell models, in an automated and highly systematic manner. HeLa Kyoto cells were grown on custom-designed micropatterned 96-well plates to facilitate spheroid assembly in the form of highly uniform arrays. Fully automated hig

SUBMITTER: Mysior MM 

PROVIDER: S-EPMC11556727 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature