Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of hiPSCs is needed. Utilizing suspension culture without microcarriers or special materials allows for massive production, automation, cost-effectiveness, and safety assurance in industrialized regenerative medicine. Here, we found that hiPSCs cultured in suspension conditions with continuous agitation without microcarriers or extracellular matrix components were more prone to spontaneous differentiation than those cultured in conventional adherent conditions. Adding PKCβ and Wnt signaling pathway inhibitors in the suspension conditions suppressed the spont

SUBMITTER: Matsuo-Takasaki M 

PROVIDER: S-EPMC11556790 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature