Exploring diabesity pathophysiology through proteomic analysis using <i>Caenorhabditis elegans</i>.

ABSTRACT:

Introduction

Diabesity, characterized by obesity-driven Type 2 diabetes mellitus (T2DM), arises from intricate genetic and environmental interplays that induce various metabolic disorders. The systemic lipid and glucose homeostasis is controlled by an intricate cross-talk of internal glucose/insulin and fatty acid molecules to maintain a steady state of internal environment.

Methods

In this study, Caenorhabditis elegans were maintained to achieve glucose concentrations resembling the hyperglycemic conditions in diabetic patients to delve into the mechanistic foundations of diabesity. Various assays were conducted to measure intracellular triglyceride levels, lifespan, pharyngeal pumping rate, oxidative stress indicators, locomotor behavior, and dopamine signaling. Pro

SUBMITTER: Subhadra M 

PROVIDER: S-EPMC11557309 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature