A cost-effective protocol for single-cell RNA sequencing of human skin.

ABSTRACT:

Introduction

Single-cell RNA sequencing (scRNAseq) and flow cytometry studies in skin are methodologically complex and costly, limiting their accessibility to researchers worldwide. Ideally, RNA and protein-based analyses should be performed on the same lesion to obtain more comprehensive data. However, current protocols generally focus on either scRNAseq or flow cytometry of healthy skin.

Methods

We present a novel label-free sample multiplexing strategy, building on the souporcell algorithm, which enables scRNAseq analysis of paired blood and skin samples. Additionally, we provide detailed instructions for simultaneous flow cytometry analysis from the same sample, with necessary adaptations for both healthy and inflamed skin specimens.

Results

This tissue multiplexi

SUBMITTER: Khoshbakht S 

PROVIDER: S-EPMC11557338 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature