GPX4 Promoter Hypermethylation Induced by Ischemia/Reperfusion Injury Regulates Hepatocytic Ferroptosis.

ABSTRACT:

Background and aims

Glutathione peroxidase 4 (GPX4) is a key factor in ferroptosis, which is involved in ischemia-reperfusion injury. However, little is known about its role in hepatic ischemia-reperfusion injury (HIRI). This study aimed to investigate the role of GPX4 methylation in ferroptosis during HIRI.

Methods

For the in vitro experiments, an oxygen and glucose deprivation cell model was established. For the in vivo experiments, an ischemia-reperfusion model was created by subjecting mice to simulated HIRI. Ferroptosis occurrence, GPX4 promoter methylation, and global methylation levels were then assessed.

Results

Ferroptosis was observed in oxygen and glucose deprivation, characterized by a significant decrease in cellular viability (P</

SUBMITTER: Bai C 

PROVIDER: S-EPMC11557362 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature