Single-cell transcriptomics reveals the molecular basis of human iPS cell differentiation into ectodermal ocular lineages.

ABSTRACT: The generation of a self-formed, ectodermal, autonomous multi-zone (SEAM) from human induced pluripotent stem cells (hiPSCs) offers a unique perspective to study the dynamics of ocular cell differentiation over time. Here, by utilising single-cell transcriptomics, we have (i) identified, (ii) molecularly characterised and (iii) ascertained the developmental trajectories of ectodermally-derived ocular cell populations which emerge within SEAMs as they form. Our analysis reveals interdependency between tissues of the early eye and delineates the sequential formation and maturation of distinct cell types over a 12-week period. We demonstrate a progression from pluripotency through to tissue specification and differentiation which encompasses both surface ectodermal and neuroectodermal ocular

SUBMITTER: Howard L 

PROVIDER: S-EPMC11557866 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature