Oriented triplex DNA as a synthetic receptor for transmembrane signal transduction.

ABSTRACT: Signal transduction across biological membranes enables cells to detect and respond to diverse chemical or physical signals, and replicating these complex biological processes through synthetic methods is of significant interest in synthetic biology. Here we present an artificial signal transduction system using oriented cholesterol-tagged triplex DNA (TD) as synthetic receptors to transmit and amplify signals across lipid bilayer membranes through H⁺-mediated TD conformational transitions from duplex to triplex. An auxiliary sequence, complementary to the third strand of the TD, ensures a controlled and preferred outward orientation of cholesterol-tagged TD on membranes. Upon external H⁺ stimuli, the conformational change triggers the translocation of the third stran

SUBMITTER: Chen H 

PROVIDER: S-EPMC11557920 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature