Biomimetic Liver Lobules from Multi-Compartmental Microfluidics.

ABSTRACT: Engineered liver lobule is highly practical in hepatic disease treatment, while constructing a 3D biomimetic lobule with a heterogeneous architecture on a large scale is challenging. Here, inspired by the natural architectural construction of hepatic lobules, biomimetic hepatic lobules are proposed with coaxially through-pores for nutrient exchange via microfluidic technology. This multi-channel microfluidic chip is made by parallelly installing capillaries. Sodium alginate (Alg) is pumped through its central channel, while Ca²⁺-loaded gelatin methacrylate (GelMA) solutions encapsulating hepatocytes, mesenchymal stem cells, and endothelia cells are pumped through surrounding channels, respectively. The rapid gelation of Alg and Ca²⁺ brings about an in situ formation o

SUBMITTER: Huang D 

PROVIDER: S-EPMC11558095 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature