PCSK7, a potential target for the treatment of age-related macular degeneration: inhibition of retinal epithelial cell death.

ABSTRACT:

Background

Age-related macular degeneration (AMD) is a complex disease with a pathophysiology that remains incompletely understood. PCSK7 is closely related to the normal development of ocular tissues; however, the roles and mechanisms of PCSK7 in AMD have yet to be elucidated. Therefore, the purpose of this study was to investigate the specific manifestations of PCSK7 in AMD.

Methods

An AMD cell model was established by using hydrogen peroxide (H₂O₂)-treated ARPE-19 cells. The efficiency of PCSK7 overexpression was analyzed by western blotting (WB) and quantitative reverse transcription PCR (RT-qPCR). Subsequently, a Cell Counting Kit 8 (CCK-8) assay was employed to assess the proliferation of ARPE-19 cells, while flow cytometry and immunofluorescence

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11558318 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature