Disturbed function of TBL1X has a differential effect on T3-regulated gene expression in two human liver cell models.

ABSTRACT:

Background

Mutations in TBL1X, part of the NCOR1/SMRT corepressor complex, were identified in patients with hereditary X-linked central congenital hypothyroidism and associated hearing loss. The role of TBL1X in thyroid hormone (TH) action, however, is incompletely understood. The aim of the present study was to investigate the role of TBL1X on T3-regulated gene expression in two human liver cell models.

Methods

A human hepatoma cell line (HepG2) wherein TBL1X was downregulated using siRNAs, and human-induced pluripotent stem cell-derived hepatocytes (iHeps) generated from individuals with a TBL1X N365Y mutation. Both cell types were treated with increasing concentrations of T3. The expression of T3-regulated genes was measured by qPCR.

Results

KLF9, CPT1A, and PCK1 m

SUBMITTER: Hu Y 

PROVIDER: S-EPMC11558919 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature