Hsa_circRNA_000166 accelerates breast cancer progression via the regulation of the miR-326/ELK1 and miR-330-5p/ELK1 axes.

ABSTRACT:

Purposes

To probe the expression, clinical significance, roles, and molecular mechanisms of circRNA_000166 in breast cancer (BC).

Methods

Clinical tissue samples were gathered from 84 BC patients who underwent surgery at the Affiliated Hospital of Chengde Medical College. Clinical data were obtained from medical records and postoperative follow-up. Expression levels of circRNA_000166, miR-326, miR-330-5p, and ELK1 mRNA in BC tissues and cells were measured by qRT-PCR, and ELK1 protein levels were assessed by WB. Pearson's correlation analysis evaluated the interrelationships between these RNAs in clinical samples. Luciferase reporter assays verified the interactions between miR-326/miR-330-5p and circRNA_000166, as well as between miR-326/miR-330-5p and ELK1. Cell proliferat

SUBMITTER: Wang MH 

PROVIDER: S-EPMC11559033 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature