Characterization and functional analysis of <i>Toxoplasma</i> Golgi-associated proteins identified by proximity labeling.

ABSTRACT: Toxoplasma gondii possesses a highly polarized secretory pathway that contains both broadly conserved eukaryotic organelles and unique apicomplexan organelles, which play essential roles in the parasite's lytic cycle. As in other eukaryotes, the T. gondii Golgi apparatus sorts and modifies proteins prior to their distribution to downstream organelles. Many of the typical trafficking factors found involved in these processes are missing from apicomplexan genomes, suggesting that these parasites have evolved unique proteins to fill these roles. Here, we identify a Golgi-localizing protein (ULP1), which is structurally similar to the eukaryotic trafficking factor p115/Uso1. We demonstrate that depletion of ULP1 leads to a dramatic reduction in parasite fitness that is the result

SUBMITTER: Pasquarelli RR 

PROVIDER: S-EPMC11559087 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature