Essential role of proline synthesis and the one-carbon metabolism pathways for systemic virulence of <i>Streptococcus pneumoniae</i>.

ABSTRACT: Virulence screens have indicated potential roles during Streptococcus pneumoniae infection for the one-carbon metabolism pathway component Fhs and proline synthesis mediated by ProABC. To define how these metabolic pathways affect S. pneumoniae virulence, we have investigated the phenotypes, transcription, and metabolic profiles of Δfhs and ΔproABC mutants. S. pneumoniae capsular serotype 6B BHN418 Δfhs and ΔproABC mutant strains had strongly reduced virulence in mouse sepsis and pneumonia models but could colonize the nasopharynx. Both mutant strains grew normally in complete media but had markedly impaired growth in chemically defined medium, human serum, and human cerebrospinal fluid. The BHN418 ΔproABC strain also had impaired gro

SUBMITTER: Ramos-Sevillano E 

PROVIDER: S-EPMC11559097 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature