N-CADHERIN⁺/CD168^- subpopulation determines therapeutic variations of UC-MSCs for cardiac repair after myocardial infarction.

ABSTRACT:

Background

The efficiency of mesenchymal stem cells (MSCs) in treating myocardial infarction (MI) remains inconsistent, which limits their therapeutic applications. Therefore, exploring the mechanism for the inconsistent efficacy of MSCs and identification the criteria for screening MSCs are important for improving the efficiency of MSCs.

Methods

Mouse model after MI was utilized to test the role of MSCs from different donors and the functional subpopulation in improving cardiac function. Heterogeneity of MSCs was identified using single-cell RNA sequencing (scRNA-seq) of MSC-GY. GSEA and Scissor analyses were used to find the functional subpopulations of MSCs that promote angiogenesis. The role of functional subpopulations in promoting angiogenesis was verified by detecting

SUBMITTER: Wu Y 

PROVIDER: S-EPMC11559175 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature