Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.

ABSTRACT:

Background

Cellular metabolism regulates the pluripotency of embryonic stem cells (ESCs). Yet, how metabolism regulates the transition among different pluripotent states remains elusive. It has been shown that protein lactylation, which uses lactate, a metabolic product of glycolysis, as a substrate, plays a critical role in various biological events. Here we focused on that glycolysis regulates the conversion between ESCs and 2-cell-like cells (2CLCs) through protein lactylation.

Methods

RNA-seq revealed the activation of 2-cell (2C) genes by suppression of Ldh. Stable isotope labeling by amino acids in cell culture (SILAC) coupled with lactylated peptide enrichment and quantitative mass spectrometric analysis was carried out to investigate the mechanism how protein lactyla

SUBMITTER: Dong Q 

PROVIDER: S-EPMC11559218 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature