Targeted nuclear degranulation of neutrophils promotes the progression of pneumonia in ulcerative colitis.

ABSTRACT:

Background

Both intestinal and pulmonary systems are parts of the mucosal immune system, comprising ∼80% of all immune cells. These immune cells migrate or are transported between various mucosal tissues to maintain tissue homeostasis.

Methods

In this study, we isolated neutrophils from the peripheral blood of patients and utilized immunofluorescence, flow cytometry, and Western blotting to confirm the incidence of "nucleus-directed degranulation" in vitro. Subsequently, we conducted a precise analysis using arivis software. Furthermore, using the DSS mouse model of colitis and tissue clearing technologies, we validated the "targeted nuclear degranulation" of neutrophils and their migration to the lungs in an inflammatory intestinal environment.

Result

In this

SUBMITTER: Shao Y 

PROVIDER: S-EPMC11560370 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature