Decreased solubility and increased adsorptivity of a biotinylated humanized anti-cocaine mAb.

ABSTRACT: Biotinylation of proteins, including antibodies, is a very useful and important modification for a variety of biochemical characterizations, including anti-drug antibody (ADA) assays used to detect antibodies raised against therapeutic antibodies. We assessed different degrees of biotin labeling of an anti-cocaine mAb currently under development for treating cocaine use disorder. We noted that higher levels of biotin labeling dramatically decreased mAb solubility, and increased the tendency to bind to surfaces, complicating characterization of the biotinylated antibody. Specifically, in phosphate buffered saline, labeling stoichiometries of more than about 3 biotin/mAb resulted in decreased recoveries due to increased binding to surfaces and decreased mAb solubility. Native gel agarose ele

SUBMITTER: Kirley TL 

PROVIDER: S-EPMC11560507 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature