Single-cell stimulus-response gene expression trajectories reveal the stimulus specificities of dynamic responses by single macrophages.

ABSTRACT: Macrophages induce the expression of hundreds of genes in response to immune threats. However, current technology limits our ability to capture single-cell inducible gene expression dynamics. Here, we generated high-resolution time series single-cell RNA sequencing (scRNA-seq) data from mouse macrophages responding to six stimuli, and imputed ensembles of real-time single-cell gene expression trajectories (scGETs). We found that dynamic information contained in scGETs substantially contributes to macrophage stimulus-response specificity (SRS). Dynamic information also identified correlations between immune response genes, indicating biological coordination. Furthermore, we showed that the microenvironmental context of polarizing cytokines profoundly affects scGETs, such that measuring resp

SUBMITTER: Sheu KM 

PROVIDER: S-EPMC11560543 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature