Structural modeling and characterization of the Mycobacterium tuberculosis MmpL3 C-terminal domain.

ABSTRACT: The Mycobacterium tuberculosis (Mtb) cell envelope provides a protective barrier against the immune response and antibiotics. The mycobacterial membrane protein large (MmpL) family of proteins export cell envelope lipids and siderophores; therefore, these proteins are important for the basic biology and pathogenicity of Mtb. In particular, MmpL3 is essential and a known drug target. Despite interest in MmpL3, the structural data in the field are incomplete. Utilizing homology modeling, AlphaFold, and biophysical techniques, we characterized the cytoplasmic C-terminal domain (CTD) of MmpL3 to better understand its structure and function. Our in silico models of the MmpL11_TB and MmpL3_TB CTD reveal notable features including a long unstructured linker that connects the g

SUBMITTER: Berkowitz N 

PROVIDER: S-EPMC11560685 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature