Exactly defined molecular weight poly(ethylene glycol) allows for facile identification of PEGylation sites on proteins.

ABSTRACT: PEGylation (the covalent attachment of one or more poly(ethylene glycol) (PEG) units to a therapeutic) is a well-established technique in the pharmaceutical industry to increase blood-residence time and decrease immunogenicity. A challenging aspect of PEGylation is the dispersity of PEGylation agents, which results in batch-to-batch variations and analytical limitations. Herein, we present an approach to overcome these limitations by manufacturing a defined molecular weight (dispersity-free) PEGylation agent. We synthesise a defined molecular weight (M_w), linear 5 kDa methoxy-PEG (mPEG) active ester in an efficient and scalable manner using an iterative liquid-phase approach based on Nanostar Sieving. We then perform a comparative study on the random PEGylation and subsequent ch

SUBMITTER: Burggraef MJ 

PROVIDER: S-EPMC11560933 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature