Effects of hap2 deletion on mnp/vp transcription in Pleurotus ostreatus grown on lignocellulosic substrates.

ABSTRACT: The regulatory mechanisms governing expression of genes encoding lignin-modifying enzymes (LME) in white-rot fungi remain largely unexplored. Although molecular cloning has identified CCAAT-boxes frequently located 5'-upstream of these genes, their role in transcriptional regulation is not well understood. This study examines the function of hap2, a gene encoding a hypothetical protein homologous to a component of the CCAAT-binding Hap complex, in the white-rot fungus Pleurotus ostreatus. Deletion of hap2 resulted in significantly reduced Mn²⁺-dependent peroxidase activity and lignin-degrading capacity compared to the parental strain 20b grown on beech wood sawdust (BWS) medium. Real-time PCR revealed that vp2 transcript levels were significantly lower in hap2 deletants than in

SUBMITTER: Kayama K 

PROVIDER: S-EPMC11561020 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature