Laminar and turbulent flow effects in high-pressure homogenization of liposomes and perfluorocarbon nanoemulsions.

ABSTRACT: Since flow characteristics are still largely unexplored for high-pressure homogenization, we investigated particle break-up at different Reynolds numbers and transition ranges in two channels (Y- and Z-channel). While the channel geometries are often treated as "black boxes", opening the channels and measuring their geometries allowed a detailed analysis of flow conditions. Transitions from laminar to turbulent flow for pressures of 250-2,000 bar have measurable effects on the sizes of perfluorocarbon (PFC)-nanoemulsion droplets emulsified by phospholipids processed simultaneously in liposomal conformation. Laminar flow has a higher size-reducing rate with growing pressure compared to turbulent flow and leads to a minimum in polydispersity. A density-driven sucrose gradient allows differen

SUBMITTER: Lubitz LJ 

PROVIDER: S-EPMC11561284 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature