Subcellular mass spectrometric detection unveils hyperglycemic memory in the diabetic heart.

ABSTRACT:

Background

Intensive glycemic control is insufficient to reduce the risk of heart failure in patients with diabetes mellitus. While the hyperglycemic memory in the diabetic cardiomyopathy has been well documented, its underlying mechanisms are not fully understood. The present study tried to investigate whether the dysregulated proteins/biological pathways, which persistently altered in diabetic hearts during normoglycemia, participate in the hyperglycemic memory.

Methods

Hearts of streptozotocin-induced diabetic mice, with or without intensive glycemic control using slow-release insulin implants, were collected. Proteins from total heart samples and subcellular fractions were assessed by mass spectrometry, Western blotting, and KEGG pathway enrichment analysis. mRNA sequenc

SUBMITTER: Zhan J 

PROVIDER: S-EPMC11561303 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature