Detection of factor Xa activity using giant magnetoresistive biosensors.

ABSTRACT:

Background

As anticoagulants are widely used to treat patients with atrial fibrillation (AF) and other thrombotic conditions, it is necessary for physicians to determine whether the medication has been taken in emergencies. Among many anticoagulants, rivaroxaban has attracted attention due to its safety and convenience. Since rivaroxaban inhibits activated coagulation factor X (factor Xa), measuring factor Xa activity can determine the presence of rivaroxaban.

Results

We report a magnetic biosensing platform capable of measuring the activity of factor Xa using peptide substrates conjugated with magnetic nanoparticles (MNPs). Due to the size of factor Xa, a new method of solution-phase assays was proposed for magnetic biosensing platforms to address issues with immobilized pe

SUBMITTER: Lee Y 

PROVIDER: S-EPMC11561370 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature