Cyanidin-3-O-glucoside ameliorates hydrogen peroxide-induced oxidative stress by regulating HMGCR-mediated cholesterol anabolism in HEK-293T cells.

ABSTRACT: Cyanidin-3-O-glucoside (C3G), as a typical anthocyanin, exhibits excellent antioxidant effects. This study aimed to demonstrate the role and mechanism of C3G in regulating 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR)-mediated cholesterol anabolism on H₂O₂-induced oxidative stress in HEK-293T cells. Firstly, the inhibitory effect of C3G on oxidative stress was confirmed by CCK-8, ROS, and mitochondrial membrane potential (MMP) experiments. Then, proteomics was used to investigate and screen differentially expressed proteins in inhibiting cellular oxidative stress by C3G. HMGCR was screened as a key differentially expressed protein by proteomic analysis. The results verified that C3G could reduce cholesterol levels by inhibiting sterol regulatory element-bind

SUBMITTER: Liu D 

PROVIDER: S-EPMC11561815 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature