Unveiling tryptophan dynamics and functions across model organisms via quantitative imaging.

ABSTRACT:

Background

Tryptophan is an essential amino acid involved in critical cellular processes in vertebrates, serving as a precursor for serotonin and kynurenine, which are key neuromodulators to influence neural and immune functions. Systematic and quantitative measurement of tryptophan is vital to understanding these processes.

Results

Here, we utilized a robust and highly responsive green ratiometric indicator for tryptophan (GRIT) to quantitatively measure tryptophan dynamics in bacteria, mitochondria of mammalian cell cultures, human serum, and intact zebrafish. At the cellular scale, these quantitative analyses uncovered differences in tryptophan dynamics across cell types and organelles. At the whole-organism scale, we revealed that inflammation-induced tryptophan concentr

SUBMITTER: Wang K 

PROVIDER: S-EPMC11562630 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature