Histological, histochemical, and immunohistochemical characterization of NANOULCOR nanostructured fibrin-agarose human cornea substitutes generated by tissue engineering.

ABSTRACT:

Background

Human artificial corneas (HAC) generated by tissue engineering recently demonstrated clinical usefulness in the management of complex corneal diseases. However, the biological mechanisms associated to their regenerative potential need to be elucidated.

Methods

In the present work, we generated HAC using nanostructured fibrin-agarose biomaterials with cultured corneal epithelial and stromal cells, and we compared the structure and histochemical and immunohistochemical profiles of HAC with control native corneas (CTR-C) and limbus (CTR-L) to determine the level of biomimicry of the HAC with these two native organs.

Results

HAC tissues consisted of a stratified epithelium and a cellular stromal substitute. The interface between stroma and epithelium was simila

SUBMITTER: Ortiz-Arrabal O 

PROVIDER: S-EPMC11562680 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature