Silencing METTL3 Increases HSP70 Expression and Alleviates Fibrosis in Keratocytes.

ABSTRACT:

Purpose

To explore the potential role of N6-methyladenosine (m6A) and its regulatory factors in corneal fibrosis response using both in vivo and in vitro models.

Methods

This study utilized the C57BL/6 mouse corneal alkali burn as an in vivo model and stimulated keratocytes with transforming growth factor beta 1 (TGF-β1) in vitro. Small interfering RNA (siRNA) was employed to downregulate the expression of YTH domain family member 2 (YTHDF2), methyltransferase-like 3 (METTL3), and fat mass and obesity-associated protein (FTO) in keratocytes. The expression of relevant genes was quantified by real-time quantitative reverse-transcription PCR (qRT-PCR), western blotting, and immunohistochemistry.

Results

After an alkali burn, m6A modification in corneas increased, with t

SUBMITTER: Jing Y 

PROVIDER: S-EPMC11562871 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature