Observing root growth and signalling responses to stress gradients and pathogens using the bi-directional dual-flow RootChip.

ABSTRACT: Plants respond to environmental stressors with adaptive changes in growth and development. Central to these responses is the role of calcium (Ca²⁺) as a key secondary messenger. Here, the bi-directional dual-flow RootChip (bi-dfRC) microfluidic platform was used to study defence signalling and root growth. By introducing salinity as sodium chloride (NaCl) treatment via a multiplexed media delivery system (MMDS), dynamic gradients were created, mimicking natural environmental fluctuations. Signal analysis in Arabidopsis thaliana plants showed that the Ca²⁺ burst indicated by the G-CaMP3 was concentration dependent. A Ca²⁺ burst initiated in response to salinity increase, specifically within the stele tissue, for 30 seconds. The signal then inte

SUBMITTER: Allan C 

PROVIDER: S-EPMC11563309 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature